| dc.contributor | Luis Armando Diaz-Torres | |
| dc.creator | María del Pilar Rodríguez-Torres | |
| dc.date | 2015-09 | |
| dc.date.accessioned | 2023-07-21T15:14:44Z | |
| dc.date.available | 2023-07-21T15:14:44Z | |
| dc.identifier | http://cio.repositorioinstitucional.mx/jspui/handle/1002/426 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7725321 | |
| dc.description | "This dissertation deals with gold nanoparticle green synthesis in aqueous solution using heparin, as a reducing and stabilizing agent, without other reagents through photochemistry, and their usage as surface enhanced Raman colloidal substrates. This work is divided in four parts: The first one is about proposing the photochemical synthesis method used to prepare the heparinbased gold nanoparticles in aqueous solution using UVA light (λ=366 nm). Two types of experiments were carried out: The nanoparticles were synthesized using reactive and pharmaceutical-grade heparins. Nanoparticle characterization was carried out with UV-Vis spectroscopy and Scanning Electron Microscopy. In the second one, a nanoparticle formation mechanism is proposed with the aid of UV-Vis and FTIR spectroscopies and pH measurements.
In the third part, surface enhanced Raman activity is investigated, finding that reactive-grade heparin gold nanoparticles yield good Raman signals and the pharmaceutical-grade ones do not. The probe molecules used were differently charged dyes: Methylene Blue (-), Rosebengal (+) and Neutral Red (+-); as well as proteins (GP120 and Bovine Serum Albumin). There are no reports on heparin-gold nanoparticles being used for this purpose to our knowledge. In the final section, this dissertation´s contributions are presented as a closure to the research that was performed." | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.relation | citation:Rodríguez-Torres (2015). "Photochemical synthesis of heparin-based gold nanoparticles and their use in Surface Enhanced Raman Spectroscopy". Tesis de Doctorado en Ciencias (Óptica). Centro de Investigaciones en Óptica, A.C. León, Guanajuato. 107 pp. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/AUTOR/UV-VIS SPECTROSCOPY, SCANNING ELECTRON MICROSCOPY, NANOPARTICLES, RAMAN SPECTROSCOPY | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.subject | info:eu-repo/classification/cti/22 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.title | PHOTOCHEMICAL SYNTHESIS OF HEPARIN-BASED GOLD NANOPARTICLES AND THEIR USE IN SURFACE ENHANCED RAMAN SPECTROSCOPY | |
| dc.type | info:eu-repo/semantics/doctoralThesis | |
| dc.coverage | León, Guanajuato | |
| dc.audience | generalPublic | |
