Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate

dc.contributorMDPI AG
dc.creatorPliego Sandoval, Jorge Enrique
dc.creatorMateos Díaz, Juan Carlos
dc.creatorRodríguez González, Jorge Alberto
dc.creatorFrancisco Valero
dc.creatorMaria del Mar Baeza Labat
dc.creatorFemat Flores, Alejandro Ricardo
dc.creatorCamacho Ruíz, Rosa María
dc.creatorSandoval Fabián, Georgina Coral
dc.creatorEnrique J Herrera López
dc.date2018-03-08T17:44:13Z
dc.date2018-03-08T17:44:13Z
dc.date2015
dc.date.accessioned2023-07-17T22:04:19Z
dc.date.available2023-07-17T22:04:19Z
dc.identifierPliego, J.; Mateos, J.C.; Rodriguez, J.; Valero, F.; Baeza, M.; Femat, R.; Camacho, R.; Sandoval, G.; Herrera-López, E.J. Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors 2015, 15, 2798-2811.
dc.identifierhttp://hdl.handle.net/11627/3420
dc.identifierhttps://dx.doi.org/10.3390%2Fs150202798
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7543902
dc.description"Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05-1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed."
dc.formatapplication/pdf
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsAcceso Abierto
dc.subjectMonitoring
dc.subjectLipase/esterase activity
dc.subjectSequential injection analysis;
dc.subjectStopped flow
dc.subjectP-nitrophenyl esters
dc.subjectELECTROQUÍMICA
dc.titleMonitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate
dc.typearticle


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