The composition of high molecular weight glutenin subunits ( HMW2GS) and low molecular weight glutenin subunits (LMW2GS) , and the presence or absence of 1BLΠ1RS translocation play an important role in determination of wheat quality1 Totally , 221 spring wheat cultivars and advanced lines were used to survey the composition of HMW2GS and LMW2GS , and the presence of 1BLΠ1RS translocation1 Effects of allelic variation in HMW2GS and LMW2GS and 1BLΠ1RS translocation on quality traits were investigated in 104 cultivars and lines1 The results showed that subunits 1 , 7 + 9 , 5 + 10 , GluA3a and GluB3j took dominant positions with frequencies of 5715 %, 4512 % , 6318 % , 2910 % and 4215 % , respectively, and 4413 % of tested genotypes from Northwestern Spring Wheat Region ( NWSWR) and 3412 % from Northeastern Spring Wheat Region (NESWR) carried 1BΠ1R translocation (Table 1) 1 Allelic variation at Glu21 and Glu23 loci had significant influence on SDS sedimentation volume , and mixing time and mixing tolerance at 1 probability level, and on kernel protein content and relative insoluble glutenin content at 5 % significant level (Table 2) 1 According to the allele contribution to mixing time and mixing tolerance , the glutenin subunit loci were ranked as : Glu2D1 > Glu2B3 > Glu2B1 > Glu2A1 > Glu2A3 ( Table 3) . Various individual glutenin alleles had significant effect on quality traits, specially on gluten strengthen related parameters1 They were ranked as : at Glu2A1 loci ,1 ,2 3 > N , at Glu2D1 ,5 + 10 > 2 + 12 , at Glu2A3 , Glu2A3d > Glu2A3b > Glu2A3c > Glu2A3a ,at Glu2B3 , Glu2B3f > Glu2B3b > Glu2B3j (Table 3). The contribution of subunits 1, 2 3, 5 + 10 , Glu2A3d , Glu2B3f to mixing time and mixing tolerance are significantly larger than their counterpart allelic variations1 The presence of 1BΠ1R translocation had a strong and negative effect on mixing time1 The mixing time of non21BLΠ1RS cultivars and lines was 319 min while 1BLΠ1RS translocation was 315 min.414-419