Articulo
Effect of dilution rate on the release of pertussis toxin and lipopolysaccharide of Bordetella pertussis
Registro en:
issn:0169-4146
issn:1476-5535
issn:1367-5435
Autor
Rodríguez, María Eugenia
Hozbor, Daniela Flavia
Samo, Analia L.
Ertola, Rodolfo J.
Yantorno, Osvaldo Miguel
Institución
Resumen
The kinetics of Bordetella pertussis growth was studied in a glutamate-limited continuous culture. Growth kinetics corresponded to Monod's model. The saturation constant and maximum specific growth rate were estimated as well as the energetic parameters, theoretical yield of cells and maintenance coefficient. Release of pertussis toxin (PT) and lipopolysaccharide (LPS) were growth-associated. In addition, they showed a linear relationship between them. Growth rate affected neither outer membrane proteins nor the cell-bound LPS pattern. Centro de Investigación y Desarrollo en Fermentaciones Industriales