During the 2000–2001 breeding season in Hope Bay, Antarctica, two adult Adelie penguins were found dead with lesions compatible with subcutaneous clostridial infection. <i>Clostridium cadaveris</i> was isolated from the musculature and the subcutaneous tissue of one of these two penguins, whereas <i>Clostridium sporogenes</i> was isolated from the subcutaneous tissue of the other penguin. <i>Escherichia coli</i> and <i>Staphylococcus</i> spp. were isolated from both animals. This is the first report of subcutaneous clostridial infection in Antarctic Adelie penguins.Facultad de Ciencias VeterinariasLaboratorio de Diagnóstico e Investigaciones Bacteriológicas