The aim of this study was to determine the prevalence of bovine viral diarrhoea (BVD), bovine neosporosis (NEOS), enzootic bovine leukosis (EBL) and bovine paratuberculosis (BP) in dual-purpose cows reared under low-tropic conditions. The study was carried out at La Pintada farm located in the municipality of Puerto Boyacá, Boyacá, Colombia, taking blood samples from 840 2–3-year-old cows collected from various departments of the country. Competitive (BVD, NEOS, EBL) and indirect (BP) ELISA tests were performed. A seropositivity of 25.83% to BVD, 16.29% to NEOS, 14.64% to EBL and 4.17% to BP was found.El objetivo de este trabajo fue determinar la prevalencia de diarrea viral bovina (DVB), neosporosis bovina (NEOS), leucosis bovina enzoótica (LBE) y paratuberculosis bovina (PB) en vacas de doble propósito criadas bajo condiciones de trópico bajo. El estudio se desarrolló en la finca La Pintada ubicada en el municipio de Puerto Boyacá, Boyacá, Colombia, tomándose muestras de sangre a 840 vacas de 2-3 años procedentes de varios departamentos del país. Se realizaron pruebas de ELISA competitiva (DVB, NEOS, LBE) e indirecta (PB). Se encontró una seropositividad de 25.83% a DVB, 19.29% a NEOS, 14.64% a LBE y 4.17% a PB.