Article
Genetic diversity and population structure of teosinte
Fecha
2008Autor
Pfaller, M.A.
Diekema, D.J.
Gibbs, D.L.
Newell, V.A.
Ng, K.P.
Colombo, A.
Finquelievich, J.
Barnes, R.
Wadula, J.
Finquelievich, J.
Tiraboschi, N.
Ellis, D.
Fameree, D.
van den Abeele, A.-M.
Senterre, J.-M.
Lopez Colombo, A.
Rennie, R.
Sanche, S.
Bijie, H.
Xu, Y.
Fu, W.
Zhong, N.S.
Rivas, P.
de Bedout, C.
Mendez, M.
Vega, R.
Mallatova, N.
Dobiasova, S.
Ayabaca, J.
Zurita, J.
Mallie, M.
Institución
Resumen
We examined data from the ARTEMIS DISK Antifungal Surveillance Program to describe geographic and temporal trends in the isolation of Candida parapsilosis from clinical specimens and the in vitro susceptibilities of 9,371 isolates to fluconazole and voriconazole. We also report the in vitro susceptibility of bloodstream infection (BSI) isolates of C. parapsilosis to the echinocandins, anidulafungin, caspofungin, and micafungin. C. parapsilosis represented 6.6% of the 141,383 isolates of Candida collected from 2001 to 2005 and was most common among isolates from North America (14.3%) and Latin America (9.9%). High levels of susceptibility to both fluconazole (90.8 to 95.8%) and voriconazole (95.3 to 98.1%) were observed in all geographic regions with the exception of the Africa and Middle East region (79.3 and 85.8% susceptible to fluconazole and voriconazole, respectively). C. parapsilosis was most often isolated from blood and skin and/or soft tissue specimens and from patients hospitalized in the medical, surgical, intensive care unit (ICU) and dermatology services. Notably, isolates from the surgical ICU were the least susceptible to fluconazole (86.3%). There was no evidence of increasing azole resistance over time among C. parapsilosis isolates tested from 2001 to 2005. Of BSI isolates tested against the three echinocandins, 92, 99, and 100% were inhibited by concentrations of ?2 ?g/ml of anidulafungin (621 isolates tested), caspofungin (1,447 isolates tested), and micafungin (539 isolates tested), respectively. C. parapsilosis is a ubiquitous pathogen that remains susceptible to the azoles and echinocandins; however, both the frequency of isolation and the resistance of C. parapsilosis to fluconazole and voriconazole may vary by geographic region and clinical service. Copyright " 2008, American Society for Microbiology. All Rights Reserved.",,,,,,"10.1128/JCM.02122-07",,,"http://hdl.handle.net/20.500.12104/41744","http://www.scopus.com/inward/record.url?eid=2-s2.0-40749148116&partnerID=40&md5=9835815df8990f312e2e431e71b96e85",,,,,,"3",,"Journal of Clinical Microbiology",,"842 849",,"46",,"Scopus",,,,,,,,,,,,"Geographic and temporal trends in isolation and antifungal susceptibility of Candida parapsilosis: A global assessment from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to 2005",,"Article"
"43474","123456789/35008",,"Fukunaga, K., Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, United States, International Research Center for Japanese Studies, 3-2 Oeyama-cho, Goryo, Nishikyo-ku, Kyoto 610-1192, Japan; Hill, J., Department of Plant Biology, University of Minnesota, St. Paul, MN 55108, United States; Vigouroux, Y., Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, United States, Institut de Recherche Pour le Developpement, Montpellier 34730, France; Matsuoka, Y., Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, United States, Fukui Prefectural University, Matsuoka-cho, Yoshida-gun, Fukui 910-1195, Japan; Sanchez G., J., Centro Universitario de Ciencias Biologicas Y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco, Mexico CP45110, Mexico; Liu, K., Statistics Department, North Carolina State University, Raleigh, NC 27695, United States, National Institute of Statistical Sciences, 19 T. W. Alexander Dr., Res. Triangle Park, NC 27709-4006, United States; Buckler, E.S., USDA-ARS, Institute for Genomic Diversity, Cornell University, Ithaca, NY 14853, United States; Doebley, J., Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, United States, Laboratory of Genetics, University of Wisconsin, 445 Henry Mall, Madison, WI 53706, United States",,"Fukunaga, K. Hill, J. Vigouroux, Y. Matsuoka, Y. Sanchez, G., J. Liu, K. Buckler, E.S. Doebley, J.",,"2005",,"The teosintes, the closest wild relatives of maize, are important resources for the study of maize genetics and evolution and for plant breeding. We genotyped 237 individual teosinte plants for 93 microsatellites. Phylogenetic relationships among species and subspecific taxa were largely consistent with prior analyses for other types of molecular markers. Plants of all species formed monophyletic clades, although relationships among species were not fully resolved. Phylogenetic analysis indicated that the Mexican annual teosintes divide into two clusters that largely correspond to the previously defined subspecies, Z. mays ssp. parviglumis and ssp. mexicana, although there are a few samples that represent either evolutionary intermediates or hybrids between these two subspecies. The Mexican annual teosintes show genetic substructuring along geographic lines. Hybridization or introgression between some teosintes and maize occurs at a low level and appears most common with Z. mays ssp. mexicana. Phylogeographic and phylogenetic analyses of the Mexican annual teosintes indicated that ssp. parviglumis diversified in the eastern part of its distribution and spread from east to west and that ssp. mexicana diversified in the Central Plateau of Mexico and spread along multiple paths to the north and east. We defined core sets of collections of Z. mays ssp. mexicana and ssp. parviglumis that attempt to capture the maximum number of microsatellite alleles for given sample sizes. Copyright " 2005 by the Genetics Society of America.