Article
Expression of autocrine prolactin and the short isoform of prolactin receptor are associated with inflammatory response and apoptosis in monocytes stimulated with Mycobacterium bovis proteins
Fecha
2007Registro en:
10.1016/j.yexmp.2015.03.016
Autor
Michel, C.R.
Delgado, E.
Martinez, A.H.
Institución
Resumen
Polycrystalline powders of bismuth cobaltite, Bi10Co16O38, with sillenite-type structure were prepared by solid-state reaction, solution and solution-polymerization methods, followed by calcination at 600 °C. By the solution-polymerization route, an aqueous solution containing metal nitrates, poly(vinyl alcohol) and sucrose was used to create a polymeric chain with the cations homogeneously distributed. After calcination, from X-ray powder diffraction patterns, an orthorhombic structure with space group Pmmm and cell parameters a = 7.2074 , b = 7.1497 and c = 5.0912 were calculated. The morphological observation of the samples by electron microscopy showed that the powders prepared by the solid-state reaction and solution processes had micron-sized grains, whereas by the solution-polymerization method, a nanostructured powder with an average particle size of 33 nm was obtained. Electrical measurements were performed on sintered thick-films of the powders obtained by the three synthesis routes, exhibiting a p-type semiconductor behavior. The gas responses of the Bi10Co16O38 sensors to O2 and CO2 were markedly improved with the nanostructured film. Besides, the dynamic change of resistance at 425 °C showed an increase of about 103 ? when exposed to CO2, suggesting that the nanostructured Bi10Co16O38 is a good candidate for environmental gas sensing. " 2007 Elsevier B.V. All rights reserved.",,,,,,"10.1016/j.snb.2007.02.031",,,"http://hdl.handle.net/20.500.12104/41362","http://www.scopus.com/inward/record.url?eid=2-s2.0-34447641609&partnerID=40&md5=5da96ba5e160e0e813015272283c3b4b",,,,,,"2",,"Sensors and Actuators, B: Chemical",,"389 395",,"125",,"Scopus WOS",,,,,,"Bismuth cobaltite; CO2 gas sensor; Nanostructured oxide; Solution-polymerization",,,,,,"Evidence of improvement in gas sensing properties of nanostructured bismuth cobaltite prepared by solution-polymerization method",,"Article"
"43185","123456789/35008",,"López-Rincón, G., Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ)Guadalajara, Mexico, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (UNAM)Mexico City, Mexico, Departamento de Investigación y Desarrollo, Laboratorios Virbac América Latina, GuadalajaraJalisco, Mexico; Mancilla, R., Instituto de Investigaciones Biomédicas (IIB), UNAMMexico City, Mexico; Pereira-Suárez, A.L., Departamento de Fisiología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG)Jalisco, Mexico; Martínez-Neri, P.A., Departamento de Fisiología, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG)Jalisco, Mexico; Ochoa-Zarzosa, A., Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo (UMSNH)Tarímbaro, Mexico; Muñoz-Valle, J.F., Instituto de Investigación en Ciencias Biomédicas (IICB), CUCS, UdeGJalisco, Mexico; Estrada-Chávez, C., Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ)Guadalajara, Mexico",,"Lopez-Rincon, G. Mancilla, R. Pereira-Suarez, A.L. Martinez-Neri, P.A. Ochoa-Zarzosa, A. Munoz-Valle, J.F. Estrada-Chavez, C.",,"2015",,"Increased levels of prolactin (PRL) have recently been associated with carcinogenesis and the exacerbation of autoimmune diseases, and might be involved in the progression of tuberculosis (TB). To investigate the relationship between PRL and prolactin receptor (PRLr) expression with inflammatory response and apoptosis in monocytes, we used THP-1 cells stimulated with antigens of the Mycobacterium bovis AN5 strain culture filtrate protein (CFP- M. bovis). Western blot (WB), real-time Polymerase chain reaction (PCR), and immunocytochemistry were performed to identify both PRL and PRLr molecules. PRL bioactivity and proinflammatory cytokine detection were assessed. The results showed that PRL and PRLr messenger RNA (mRNA) were synthesized in THP-1 monocytes induced with CFP- M. bovis at peaks of 176- and 404-fold, respectively. PRL forms of 60 and 80. kDa and PRLr isoforms of 40, 50, and 65. kDa were also identified as time-dependent, while 60-kDa PRL, as well as 40-, and 50-kDa PRLr, were found as soluble forms in culture media and later in the nucleus of THP-1 monocytes. PRL of 60. kDa released by monocytes exhibited bioactivity in Nb2 cells, and both synthesized PRL and synthesized PRLr were related with nitrite and proinflammatory cytokine levels proapoptotic activity in CFP- M. bovis-induced monocytes. Our results suggest the overexpression of a full-autocrine loop of PRL and PRLr in monocytes that enhances the inflammatory response and apoptosis after priming with M. bovis antigens. " 2015 Elsevier Inc.