Artigo de Periódico
Estabelecimento in vitro de Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: uma espécie endêmica do vale do rio São Francisco
Fecha
2020Autor
Anna Luiza Mota Docha
Leandro Silva de Oliveira
Nayara dos Santos Souza
Gilvano Ebling Brondani
Institución
Resumen
Ceiba rubriflora is a native Brazilian species, critically endangered, from region of middle São Francisco River. C. rubriflora’s seed production is scarce because of its endemism. Therefore, the micropropagation is presented as a tool for its propagation and seedling production. Thus, the aim of the present study was to evaluate the in vitro establishment of C. rubriflora seeds after disinfestation with different concentrations of active chlorine and testing the in vitro multiplication of apical shoots in different concentrations of 6-benzylaminopurine (BAP). The in vitro establishment of seeds was performed in MS culture medium, which were previously submitted to sodium hypochlorite (NaOCl) disinfection at concentrations of 0.0; 0.5; 1.0; 1.5; and 2.5% active chlorine. Apical shoots of in vitro germinated seedlings were used as explants for multiplication, in MS culture medium, supplemented with BAP in concentrations of 0.0; 1.0 and 2.0 mg L-1. The evaluations were related to seedling survival, fungal and bacterial contamination and number of shoots per explant. The in vitro germination of seeds was satisfactory, with survival rate higher than 98%.
The percentage of in vitro contamination was only 2%, free from bacterial cultures. The apical shoots of C. rubriflora
were not multiplied in a single subculture in vitro in BAP culture medium. The results demonstrate the viability of
in vitro germination of C. rubriflora seeds for the introduction and subsequent micropropagation process, opening
perspectives for further studies with the species.