Comportamiento de células STEM Mesenquimales aisladas de Papila Apical y Pulpa Sana Tras Intercambio de sus medios condicionados
Fecha
2017-12-19Registro en:
Buitrago Ayala, J. V., Bustos Valero, Y.A., Lamprea Sierra, J.A., Pulido Rangel, L. M. y Sterling Bautista, J. (2017). Comportamiento de células STEM Mesenquimales aisladas de Papila Apical y Pulpa Sana Tras Intercambio de sus medios condicionados [Tesis de Especialización]. Universidad Santo Tomás. Bucaramanga, Colombia
reponame:Repositorio Institucional Universidad Santo Tomás
instname:Universidad Santo Tomás
Autor
Buitrago Ayala, Jeimi Viviana
Bustos Valero, Yury Andrea
Lamprea Sierra, July Alexandra
Pulido Rangel, Lina Marcela
Sterling Bautista, Jennifer
Institución
Resumen
The last decade has been deeply marked by persistent attempts to use mesenchymal stem cells (MSCs) ex vivo expanded and manipulated, as a tool in different types of regenerative therapy. Objective: To evaluate the proliferation, viability and differentiation potential after conditioned media exhange obtained from mesenchymal stem cells isolated from the third molar apical papilla and healthy premolar pulp. Results: It was posible to isolate Mesenchymal stem cells from apical papilla of third molars and healthy premolar pulp. 37 samples were collected, 16 (43.3%) were papillae and 21 (56.7%) were pulp. A growth rate of 24.73 was observed. All the isolates that grew took an average of 22.2 days to reach confluence. It was obtained 9 conditioned medias, 4 of papila and 5 of pulp. The percentage of differentiation towards osteocytes was between 70% and 50% and for adipocytes was less than 30%, after the exchange. Conclusions: The results of the study provide evidence that the premolar dental pulp and papilla from third molars contain a population of multipotent stem cells. These stem cells can be isolated and expanded in vitro, in addition, their proliferation, viability and differentiation potential towards osteocytes vary as they are cultivated after the conditionated media Exchange regarding to the non-exchanged cells.