Artículo
Astrocyte-Derived Small Extracellular Vesicles Regulate Dendritic Complexity through miR-26a-5p Activity
Fecha
2020-04-10Autor
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Universidad San Sebastián
Luarte, Alejandro
Henzi, Roberto
Fernández, Anllely
Gaete, Diego
Cisternas, Pablo
Pizarro, Matias
Batiz, Luis Federico
Villalobos, Isabel
Masalleras, Matias
Vergara, Rodrigo
Varas-Godoy, Manuel
Abarzua-Catalan, Lorena
Herrera-Molina, Rodrigo
Lafourcade, Carlos
Wyneken, Ursula
Institución
Resumen
In the last few decades, it has been established that astrocytes play key roles in the regulation of neuronal morphology. However, the contribution of astrocyte-derived small extracellular vesicles (sEVs) to morphological differentiation of neurons has only recently been addressed. Here, we showed that cultured astrocytes expressing a GFP-tagged version of the stress-regulated astrocytic enzyme Aldolase C (Aldo C-GFP) release small extracellular vesicles (sEVs) that are transferred into cultured hippocampal neurons. Surprisingly, Aldo C-GFP-containing sEVs (Aldo C-GFP sEVs) displayed an exacerbated capacity to reduce the dendritic complexity in developing hippocampal neurons compared to sEVs derived from control (i.e., GFP-expressing) astrocytes. Using bioinformatics and biochemical tools, we found that the total content of overexpressed Aldo C-GFP correlates with an increased content of endogenous miRNA-26a-5p in both total astrocyte homogenates and sEVs. Notably, neurons magnetofected with a nucleotide sequence that mimics endogenous miRNA-26a-5p (mimic 26a-5p) not only decreased the levels of neuronal proteins associated to morphogenesis regulation, but also reproduced morphological changes induced by Aldo-C-GFP sEVs. Furthermore, neurons magnetofected with a sequence targeting miRNA-26a-5p (antago 26a-5p) were largely resistant to Aldo C-GFP sEVs. Our results support a novel and complex level of astrocyte-to-neuron communication mediated by astrocyte-derived sEVs and the activity of their miRNA content.
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Guía infográfica de las aves del campus de la Pontificia Universidad Javeriana : Sede central
Echeverry-Galvis, Maria Angela; Cortés Cano, Juan Sebastián; Juan Sebastián, Sergio Andrés; Zapata Montoya, Sebastián; Botiva, Daniel Yesid; Linero, Daniela; Orozco, Daniela; Medina, Juan Sebastián; Sánchez, Juan Sebastián; Zuluaga, Juliana; Gómez, Laura María; Erazo, Paula Andrea; Ortegón, Salomé; Niño, Stephanie; Bocanegra, Valentina; Cardona, Valentina; Rincón, Víctor Julio; Moreno, Johan Sebastian; Jiménez, ValeriaEn las áreas urbanas las aves les dan a los ciudadanos un contacto directo con la vida silvestre. Su presencia es esencial para la polinización de jardines y huertas urbanas, para el control de poblaciones de roedores y ... -
Guía infográfica de las aves del campus de la Pontificia Universidad Javeriana : Sede central
Echeverry-Galvis, Maria Angela; Cortés Cano, Juan Sebastián; Juan Sebastián, Sergio Andrés; Zapata Montoya, Sebastián; Botiva, Daniel Yesid; Linero, Daniela; Orozco, Daniela; Medina, Juan Sebastián; Sánchez, Juan Sebastián; Zuluaga, Juliana; Gómez, Laura María; Erazo, Paula Andrea; Ortegón, Salomé; Niño, Stephanie; Bocanegra, Valentina; Cardona, Valentina; Rincón, Víctor Julio; Moreno, Johan Sebastian; Jiménez, ValeriaEn las áreas urbanas las aves les dan a los ciudadanos un contacto directo con la vida silvestre. Su presencia es esencial para la polinización de jardines y huertas urbanas, para el control de poblaciones de roedores y ... -
Niveles de empatía en estudiantes de Odontología de la Universidad San Sebastián, Concepción, Chile
Universidad San Sebastián; Universidad San Sebastián; Universidad San Sebastián; Torres-Martínez, Pilar Alejandra; Barrios-Penna, Carolina Alejandra; Fonseca-Molina, Juan Fernando; Díaz-Narváez, Víctor Patricio; González-Cruz, Sebastián Alejandro (2017)Introduction: The training of future dentists should consider developing empathy while they are still students. The first step to consider such training is to assess empathic behavior. Objective: To measure levels of empathy ...