info:ar-repo/semantics/artículo
TLR activation, immune response and viral protection elicited in cattle by a commercial vaccine against Bovine Herpesvirus-1
Fecha
2022-01Autor
Kornuta, Claudia Alejandra
Cheuquepan Valenzuel, Felipe Andres
Bidart, , Juan Esteban
Soria, Ivana
Gammella, Mariela
Quattrocchi, Valeria
Hecker, Yanina
Moore, Prando Dadin
Romera, Sonia Alejandra
Marin, Maia Solange
Zamorano, Patricia Ines
Langellotti, Cecilia Ana
Resumen
The innate and acquired immune response induced by a commercial inactivated vaccine against Bovine Herpesvirus-1 (BoHV-1) and protection conferred against the virus were analyzed in cattle. Vaccination induced high levels of BoHV-1 antibodies at 30, 60, and 90 days post-vaccination (dpv). IgG1 and IgG2 isotypes were detected at 90 dpv, as well as virus-neutralizing antibodies. An increase of anti-BoHV-1 IgG1 in nasal swabs was detected 6 days post-challenge in vaccinated animals. After viral challenge, lower virus excretion and lower clinical score were observed in vaccinated as compared to unvaccinated animals, as well as BoHV-1-specific proliferation of lymphocytes and production of IFNγ, TNFα, and IL-4. Downregulation of the expression of endosome Toll-like receptors 8–9 was detected after booster vaccination. This is the first thorough study of the immunity generated by a commercial vaccine against BoHV-1 in cattle.