dc.creator | Maroniche, Guillermo Andrés | |
dc.creator | Mongelli, Vanesa Claudia | |
dc.creator | Llauger, Gabriela | |
dc.creator | Alfonso, Victoria | |
dc.creator | Taboga, Oscar Alberto | |
dc.creator | Del Vas, Mariana | |
dc.date.accessioned | 2019-06-13T13:00:45Z | |
dc.date.accessioned | 2023-03-15T14:00:08Z | |
dc.date.available | 2019-06-13T13:00:45Z | |
dc.date.available | 2023-03-15T14:00:08Z | |
dc.date.created | 2019-06-13T13:00:45Z | |
dc.date.issued | 2012-09 | |
dc.identifier | 0042-6822 | |
dc.identifier | https://doi.org/10.1016/j.virol.2012.04.016 | |
dc.identifier | https://www.sciencedirect.com/science/article/pii/S0042682212002115 | |
dc.identifier | http://hdl.handle.net/20.500.12123/5308 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/6208466 | |
dc.description.abstract | The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and α-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread. | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Virology 430 (2) : 81-89 (September 2012) | |
dc.subject | Virus de las Plantas | |
dc.subject | Enfermedades de las Plantas | |
dc.subject | Proteínas | |
dc.subject | Experimentación in Vivo | |
dc.subject | Plant Viruses | |
dc.subject | Plant Diseases | |
dc.subject | Proteins | |
dc.subject | In Vivo Experimentation | |
dc.title | In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |