dc.creatorBellido, Demian
dc.creatorChacana, Pablo
dc.creatorMozgovoj, Marina Valeria
dc.creatorGonzalez, Diego
dc.creatorGoldbaum, Fernando Alberto
dc.creatorWigdorovitz, Andres
dc.creatorDus Santos, Maria Jose
dc.date.accessioned2019-01-28T15:48:56Z
dc.date.accessioned2023-03-15T13:58:09Z
dc.date.available2019-01-28T15:48:56Z
dc.date.available2023-03-15T13:58:09Z
dc.date.created2019-01-28T15:48:56Z
dc.date.issued2012-02
dc.identifier2156-8456
dc.identifier2156-8502
dc.identifierhttp://dx.doi.org/10.4236/abb.2012.31012
dc.identifierhttps://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200
dc.identifierhttp://hdl.handle.net/20.500.12123/4341
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6207543
dc.description.abstractLumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology.
dc.languageeng
dc.publisherScientific Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceAdvances in Bioscience and Biotechnology 3 (1) : 80-86 (February 2012)
dc.subjectBrucella
dc.subjectInmunogenética
dc.subjectProteínas
dc.subjectYema de Huevo
dc.subjectRotavirus Bovino
dc.subjectAnticuerpos
dc.subjectImmunogenetics
dc.subjectProteins
dc.subjectEgg Yolk
dc.subjectBovine Rotavirus
dc.subjectAntibodies
dc.titleBrucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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