info:ar-repo/semantics/artículo
Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
Fecha
2016Autor
Reyes Martinez, Carina Andrea
de Francesco, Agustina
Ocolotobiche, Eliana Evelina
Costa, Norma Beatriz
Garcia, Maria Laura
Resumen
Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants
were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to
expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they
showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the
recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with
Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific
action of 24K-derived hairpin over CPsV multiplication.