info:ar-repo/semantics/artículo
Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
Fecha
2015Autor
Mondino, Eduardo Ariel
Covacevich, Fernanda
Studdert, Guillermo
Pimentel, João Pedro
Berbara, Ricardo Luiz Louro
Resumen
We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and
cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of
nematodes. Nematodes were isolated from an agricultural area under different management of long-term
crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain
Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The
CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful
method because positive amplifications were obtained by using both eukaryotic and nematode specific
primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.