Escherichia coli isolated from feces of diarrheic piglets. Patterns of adherence to HEp-2 cells
Escherichia coli aislada de fecas de cerditos diarreicos. Patrones de adherencia a células HEp-2
dc.creator | ZAMORA, J. | |
dc.creator | REINHARDT, G. | |
dc.creator | POLETTE, M. | |
dc.creator | ORELLANA, C. M. | |
dc.date | 2000-12-29 | |
dc.date.accessioned | 2022-12-20T20:05:54Z | |
dc.date.available | 2022-12-20T20:05:54Z | |
dc.identifier | http://revistas.uach.cl/index.php/amv/article/view/4598 | |
dc.identifier | 10.4067/S0301-732X2000000200013 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5426095 | |
dc.description | Diarrheic feces from 150 piglets younger than 40 days old, were examined in order to isolate E. coli strains and test their adherence capacity to HEp-2 cells. For this purpose, samples were seeded on eosin methylen blue agar. Isolated E. coli strains were cultured in tryptone broth with 1% D-mannose and then inoculated to HEp-2 monolayer cells.. Strains with adherence capacity were obtained from 20 piglets. In 11 piglets (7.3%) the isolated strains showed localised adherence; in 5 piglets (3.3, they had an aggregative pattern of adherence and in 4 piglets (2.7%), they showed a pattern of diffuse adherence. | en-US |
dc.description | Se examinaron fecas diarreicas de 150 cerditos menores de 40 días de edad con el propósito de aislar cepas de E. coli y probar su capacidad de adherirse a las células HEp-2. Para tal objeto, las muestras se sembraron en agar eosina azul de metileno. Las cepas de E. coli aisladas se sembraron en agua triptonada con 1% de D- manosa y se inocularon sobre monocapas de células HEp-2. Las cepas con adherencia correspondieron a 20 cerditos. En 11 cerditos (7,3%) las cepas aisladas exhibieron adherencia localizada, en 5 (3,3%) fueron adherencia agregativa y en 4 (2,7%) presentaron un patrón de adherencia difusa. | es-ES |
dc.language | spa | |
dc.publisher | Archivos de Medicina Veterinaria | es-ES |
dc.relation | http://revistas.uach.cl/index.php/amv/article/view/4598/6355 | |
dc.rights | Derechos de autor 2000 Archivos de Medicina Veterinaria | es-ES |
dc.source | Archivos de Medicina Veterinaria; Vol. 32 Núm. 2 (2000); 245-251 | es-ES |
dc.source | 0717-6201 | |
dc.source | 0301-732X | |
dc.subject | E. coli | es-ES |
dc.subject | LAEC | es-ES |
dc.subject | DAEC | es-ES |
dc.subject | EAggEC | es-ES |
dc.subject | diarrea | es-ES |
dc.subject | cerditos | es-ES |
dc.subject | E. coli | en-US |
dc.subject | LAEC | en-US |
dc.subject | DAEC | en-US |
dc.subject | EAggEC | en-US |
dc.subject | diarrhoea | en-US |
dc.subject | piglets | en-US |
dc.title | Escherichia coli isolated from feces of diarrheic piglets. Patterns of adherence to HEp-2 cells | en-US |
dc.title | Escherichia coli aislada de fecas de cerditos diarreicos. Patrones de adherencia a células HEp-2 | es-ES |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion |