info:eu-repo/semantics/article
Escherichia coli isolated from feces of diarrheic piglets. Patterns of adherence to HEp-2 cells
Escherichia coli aislada de fecas de cerditos diarreicos. Patrones de adherencia a células HEp-2
Registro en:
10.4067/S0301-732X2000000200013
Autor
ZAMORA, J.
REINHARDT, G.
POLETTE, M.
ORELLANA, C. M.
Institución
Resumen
Diarrheic feces from 150 piglets younger than 40 days old, were examined in order to isolate E. coli strains and test their adherence capacity to HEp-2 cells. For this purpose, samples were seeded on eosin methylen blue agar. Isolated E. coli strains were cultured in tryptone broth with 1% D-mannose and then inoculated to HEp-2 monolayer cells.. Strains with adherence capacity were obtained from 20 piglets. In 11 piglets (7.3%) the isolated strains showed localised adherence; in 5 piglets (3.3, they had an aggregative pattern of adherence and in 4 piglets (2.7%), they showed a pattern of diffuse adherence. Se examinaron fecas diarreicas de 150 cerditos menores de 40 días de edad con el propósito de aislar cepas de E. coli y probar su capacidad de adherirse a las células HEp-2. Para tal objeto, las muestras se sembraron en agar eosina azul de metileno.
Las cepas de E. coli aisladas se sembraron en agua triptonada con 1% de D- manosa y se inocularon sobre monocapas de células HEp-2.
Las cepas con adherencia correspondieron a 20 cerditos. En 11 cerditos (7,3%) las cepas aisladas exhibieron adherencia localizada, en 5 (3,3%) fueron adherencia agregativa y en 4 (2,7%) presentaron un patrón de adherencia difusa.