Artículos de revistas
MALDI-TOF MS identification of Prototheca algae associated with bovine mastitis
Fecha
2021-01-01Registro en:
Journal of Veterinary Diagnostic Investigation.
1943-4936
1040-6387
10.1177/10406387211036459
2-s2.0-85112162300
Autor
Universidade de São Paulo (USP)
University of Warsaw
Universidade Estadual Paulista (UNESP)
Institución
Resumen
We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases.