Artículos de revistas
How can micelle systems be rebuilt by a heating process?
Fecha
2012-02-10Registro en:
International Journal of Nanomedicine, v. 7, p. 141-150.
1176-9114
1178-2013
2-s2.0-84856639968
Autor
Federal University of rio grande do Norte (UFrN)
UFrN
Universidade Estadual Paulista (UNESP)
Institución
Resumen
The aim of this work was to evaluate how an aqueous micellar system containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg. L -1, 5 mg. L -1, 0.5 mg. L -1, and 0.05 mg. L -1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K +) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the effcacy of AmB-DOC and AmB-DOC-H were assessed by K + leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg. L -1). For K + leakage, both AmB-DOC and AmB-DOC-H showed a similar profle for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profle of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. © 2012 Silva-Filho et al.