Artículos de revistas
Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples
Detecção de mórulas e DNA de Ehrlichia canis em sangue e em aspirado de baço em cães naturalmente infectados
Fecha
2010-01-01Registro en:
Revista Brasileira de Parasitologia Veterinaria, v. 19, n. 2, p. 24-28, 2010.
0103-846X
2-s2.0-77954288539
Autor
Universidade de Uberaba - UNIUBE
Centro Universitário de Rio Preto - UNIRP
Universidade Estadual Paulista (UNESP)
Institución
Resumen
The aim of this study was to compare the detection of Ehrlichia canis morulae and DNA by nPCR in whole blood and spleen aspiration. The sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears. E. canis DNA was detected in 29/40 blood samples. We verified that morulae detection is more efficient in cytological preparations from spleen aspiration. On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis.