Artículos de revistas
Low-fouling properties in serum of carboxylic-oligo(ethylene glycol)-based interfaces
Fecha
2021-06-05Registro en:
Colloids And Surfaces A-physicochemical And Engineering Aspects. Amsterdam: Elsevier, v. 618, 6 p., 2021.
0927-7757
10.1016/j.colsurfa.2021.126426
WOS:000642467400002
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
In the present work we evaluated the low-fouling feature of a carboxylic-oligo(ethylene glycol) self-assembled monolayer (carboxylic-OEG-SAM) interface using a quartz crystal microbalance with dissipation monitoring (QCM-D). QCM-D measurements allowed us to estimate the amount of protein loading in two different serum dilutions at two different interfaces: bare gold and carboxylic-OEG. The observed amount of protein adsorbed onto bare gold is about twice higher that of carboxylic OEG interface, confirming the low-fouling characteristics of OEG-modified surfaces. Additionally, QCM-D results demonstrated the existence of two protein adsorption regimes, a faster and a slower, with distinct dissipation energies which was modelled by a two-step kinetic model. The faster regime was attributed to the adsorption of proteins into free sites of the carboxylic-OEG-SAM in a rigid binding process, followed by a slower and more viscoelastic adsorption process ascribed to structural conformational changes; this slower step conforms with the filling of remaining free sites associated with the steric hindrance in which protein-protein interactions defines the slower rate constant for the adsorption.