Effects of varying concentrations of alanine, glutamine, glycine and valine on amino acid transport and intracellular metabolism in mammary epithelial cells

Abstract

The objective of this study was to evaluate the effects of varying concentrations of the main non-essential amino acids (NEAA) involved in bi-directional transport, Ala, Gln, and Gly, on uptake of Val and other branched-chain amino acids (BCAA) by mammary epithelial cells. A cell culture trial was conducted with four treatments representing high and low concentrations of AA known to be involved in efflux transport. The bi-directional transport of BCAA was affected by varying Val, Ala, Gln, and Gly concentrations, indicating competition for AA transporters.