Artículos de revistas
Antimicrobial and antibiofilm activity of Lys-[Trp6]hy-a1 combined with ciprofloxacin against gram-negative bacteria
Fecha
2020-01-01Registro en:
Protein and Peptide Letters, v. 27, n. 11, p. 1124-1131, 2020.
1875-5305
0929-8665
10.2174/0929866527666200416145549
2-s2.0-85090247706
Autor
Federal University of Ceara – UFC
University Center IN-TA – UNINTA
Federal University of Jatai
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Background: Ciprofloxacin (Cip) is the most commonly used quinolone in clinical prac-tice; however large-scale use has favored the increase of multiresistant pathogenic microorganisms. Antimicrobial peptides (AMPs) appear to be a promising alternative in potentiating these conven-tional drugs. Objective: The aim of this study was to evaluate the effect of the peptide Lys-[Trp6]hy-a1 (lys-a1) on the antimicrobial and antibiofilm activity of ciprofloxacin against clinically relevant gram-nega-tive bacteria. Methods: The antimicrobial effects of Cip and lys-a1 were assessed by determining the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The synergistic action of Cip and lys-a1 was determined by checkerboard assay. The time-kill curve was con-structed for the Cip/lys-a1 combination against Pseudomonas aeruginosa ATCC 9027. The antibio-film activity of this combination was analyzed by crystal violet, colony-forming unit count and atomic force microscopy (AFM). Results: The data demonstrated that lys-a1 was able to inhibit planktonic growth of strains of P. aeruginosa and Klebsiella pneumoniae both at 125 μg/mL. The fractional inhibitory concentration index (FICi) showed a synergistic effect between Cip and lys-a1 against P. aeruginosa, decreasing the MICs of the individual antimicrobial agents by 4-and 8-fold, respectively. This effect was also observed for the death kinetics and antibiofilm activity. Analysis of the early biofilms (6 h) as well as isolated cells by AFM images evidenced the cell perturbation caused by Cip/lys-a1 treatment. Conclusion: These data suggest that lys-a1 has biotechnological potential as a therapeutic tool for the treatment of infections caused by clinically relevant microorganisms, especially P. aeruginosa.