Artículos de revistas
Heat-induced triploids in Brycon amazonicus: A strategic fish species for aquaculture and conservation
Fecha
2021-01-01Registro en:
Zygote.
1469-8730
0967-1994
10.1017/S0967199421000125
2-s2.0-85103703930
Autor
Rodovia Pref. Euberto Nemésio Pereira de Godoy
Faculdade de Medicina Veterinária e Ciência Animal
Unidade Acadêmica de Serra Talhada
Via de Acesso Prof. Paulo Donato Castellane S/n
Universidade Estadual Paulista (Unesp)
China Three Gorges Corporation (CTG)
Institución
Resumen
Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.