Artículos de revistas
Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats
Fecha
2020-01-01Registro en:
Acta Cirurgica Brasileira, v. 35, n. 8, p. 1-7, 2020.
1678-2674
0102-8650
10.1590/s0102-865020200080000005
S0102-86502020000800204
2-s2.0-85090624995
S0102-86502020000800204.pdf
Autor
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Universidade de Marília (UNIMAR)
Institución
Resumen
Purpose: To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods: Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results: The immunoreactivity of Caspases-3 and-9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions: The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.