dc.contributor | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2020-12-12T02:15:10Z | |
dc.date.accessioned | 2022-12-19T21:08:14Z | |
dc.date.available | 2020-12-12T02:15:10Z | |
dc.date.available | 2022-12-19T21:08:14Z | |
dc.date.created | 2020-12-12T02:15:10Z | |
dc.date.issued | 2020-01-01 | |
dc.identifier | Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 26. | |
dc.identifier | 1678-9199 | |
dc.identifier | 1678-9180 | |
dc.identifier | http://hdl.handle.net/11449/200754 | |
dc.identifier | 10.1590/1678-9199-JVATITD-2019-0093 | |
dc.identifier | S1678-91992020000100313 | |
dc.identifier | 2-s2.0-85087927581 | |
dc.identifier | S1678-91992020000100313.pdf | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5381388 | |
dc.description.abstract | Background: Ventral root avulsion (VRA) is an experimental approach in which there is an abrupt separation of the motor roots from the surface of the spinal cord. As a result, most of the axotomized motoneurons degenerate by the second week after injury, and the significant loss of synapses and increased glial reaction triggers a chronic inflammatory state. Pharmacological treatment associated with root reimplantation is thought to overcome the degenerative effects of VRA. Therefore, treatment with dimethyl fumarate (DMF), a drug with neuroprotective and immunomodulatory effects, in combination with a heterologous fibrin sealant/biopolymer (FS), a biological glue, may improve the regenerative response. Methods: Adult female Lewis rats were subjected to VRA of L4-L6 roots followed by reimplantation and daily treatment with DMF for four weeks. Survival times were evaluated 1, 4 or 12 weeks after surgery. Neuronal survival assessed by Nissl staining, glial reactivity (anti-GFAP for astrocytes and anti-Iba-1 for microglia) and synapse preservation (anti-VGLUT1 for glutamatergic inputs and anti-GAD65 for GABAergic inputs) evaluated by immunofluorescence, gene expression (pro- and anti-inflammatory molecules) and motor function recovery were measured. Results: Treatment with DMF at a dose of 15 mg/kg was found to be neuroprotective and immunomodulatory because it preserved motoneurons and synapses and decreased astrogliosis and microglial reactions, as well as downregulated the expression of pro-inflammatory gene transcripts. Conclusion: The pharmacological benefit was further enhanced when associated with root reimplantation with FS, in which animals recovered at least 50% of motor function, showing the efficacy of employing multiple regenerative approaches following spinal cord root injury. | |
dc.language | eng | |
dc.relation | Journal of Venomous Animals and Toxins Including Tropical Diseases | |
dc.rights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Dimethyl-fumarate | |
dc.subject | Fibrin sealant | |
dc.subject | Immunomodulation | |
dc.subject | Neuroprotection | |
dc.subject | Ventral root avulsion | |
dc.title | Neuroprotection and immunomodulation by dimethyl fumarate and a heterologous fibrin biopolymer after ventral root avulsion and reimplantation | |
dc.type | Artículos de revistas | |