Artículos de revistas
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches
Fecha
2020-06-01Registro en:
Journal of Applied Ichthyology, v. 36, n. 3, p. 339-344, 2020.
1439-0426
0175-8659
10.1111/jai.14025
2-s2.0-85081726801
Autor
Chico Mendes Institute of Biodiversity Conservation
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Federal Rural University of Rio de Janeiro
Institución
Resumen
The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies.