Artículos de revistas
Effect of EDTA and QMIX ultrasonic activation on the reduction of microorganisms and endotoxins in ex vivo human root canals
Fecha
2019-05-01Registro en:
Brazilian Dental Journal, v. 30, n. 3, p. 220-226, 2019.
1806-4760
0103-6440
10.1590/0103-6440201902470
S0103-64402019000300220
2-s2.0-85067513563
S0103-64402019000300220.pdf
9304600166583100
0000-0003-0987-5594
Autor
Universidade Estadual Paulista (Unesp)
School of Dentistry
Institución
Resumen
The present study aimed to compare the effectiveness of QMiX and 17% EDTA associated to passive ultrasonic irrigation (PUI) or manual agitation (MA) on the reduction of E. faecalis, E. coli and LPS from root canals. Forty single rooted human teeth were randomly divided into fourgroups(n=10),according to the final irrigation protocol: EDTA+MA,QMiX+MA,EDTA+PUI, QMiX+PUI. Sample collections were obtained from the root canal content immediately before preparation (baseline-S1), after instrumentation (S2), after final irrigation protocol (S3) and 7 days after instrumentation and final irrigation (S4). The antimicrobial effectivity and on endotoxin content were analyzed by culture procedure (CFU/mL) and LAL assay (EU/ mL), respectively. The results were statistically analyzed by Kruskal-Wallis and Friedman test (α=5%). QMiX+MA and QMiX+PUI reduced 100% of E. coli and E. faecalis bacteria and also preventedE. faecalis regrowthat S4. EDTAsignificantly reducedE. coli, but itwas not effective in reducing E. faecalis. All protocols reduced EU/mL when compared to S1, however at S4 there was a significant reduction of EU/mL only in the QMiX+MA and QMiX+PUI groups in relation to S3 and S2, respectively. Final irrigation with QMiX associated with MA or PUI had superior antibacterial efficacy compared to EDTA, eliminating 100% of E. coli and E. faecalis strains. In addition, QMiX+PUI reduced 97.61% of the initial content of LPS.