Artículos de revistas
Glufosinate Resistance Level is Proportional to Phosphinothricin Acetyltransferase Gene Expression in Glufosinate-Resistant Maize
Fecha
2018-12-05Registro en:
Journal of Agricultural and Food Chemistry, v. 66, n. 48, p. 12641-12650, 2018.
1520-5118
0021-8561
10.1021/acs.jafc.8b04823
2-s2.0-85058021929
9855493448161702
0000-0003-0431-5942
Autor
Universidade Estadual Paulista (Unesp)
Universidade Federal do Paraná (UFPR)
Institución
Resumen
Phosphinothricin acetyltransferase (pat) gene confers resistance to glufosinate by transforming this herbicide into N-acetyl-l-glufosinate (NAG). The pat gene was inserted in six maize hybrids (Herculex, Agrisure TL, Herculex Yieldgard, Leptra, Viptera 3, Power Core) as a selectable marker, and its expression was evaluated by qPCR in comparison with the maize glufosinate-susceptible cultivar VTPRO. In addition, the levels of NAG, glufosinate degradation, ammonia accumulation, electron transport rate (ETR), visual injury, and biomass were also investigated. The VTPRO, Herculex, Agrisure, and Viptera showed lower pat gene expression, and consequently lower NAG contents and glufosinate degradation, as well as reduced ETR and biomass accumulation. In contrast, greater ammonia accumulation and higher visual injury were observed. The ranking of pat gene expression was Leptra > Power Core > Herculex Yieldgard ≫ Herculex > Agrisure TL = Viptera 3 > VTPRO. This gene expression was proportional to the glufosinate resistance level observed in each maize hybrid.