Artículos de revistas
Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus
Fecha
2019-06-01Registro en:
Archives of Virology, v. 164, n. 6, p. 1677-1682, 2019.
0304-8608
10.1007/s00705-019-04214-z
2-s2.0-85064200276
7179273060624761
Autor
UnB-University of Brasília
Federal University of Santa Maria
Universidade Estadual Paulista (Unesp)
Institución
Resumen
We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.