Artículos de revistas
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse
Fecha
2018-07-01Registro en:
Pesquisa Veterinaria Brasileira, v. 38, n. 7, p. 1300-1306, 2018.
1678-5150
0100-736X
10.1590/1678-5150-PVB-5211
S0100-736X2018000701300
2-s2.0-85055327272
S0100-736X2018000701300.pdf
8502462873517464
0000-0003-4939-8024
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively.
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