The aim of this study was to evaluate the effect of humectants used in cosmetic products in improving skin hydration and the efficiency of the skin barrier. The effect of Propanediol in different concentrations (5%, 10% and 15%), applied alone or combined with Butylene glycol (5%) and/or Glycerol (5%), was evaluated throughout the study. Moisturizing effect was assessed through skin capacitance measurements using the Corneometer® 825 probe and skin barrier function was assessed through skin water loss (TEWL) measurements using the Tewameter® TM300 probe. Capacitance and TEWL measurements were performed at baseline, after 15 minutes, 2 and 8 hours of products application. The results obtained in this study can be used in the development of new formulations and to conceive prescription forms that aim for better clinical answers to repair and hydrate the skin.