Lippia origanoides supercritical fluid extract and its major constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and modulate cell division in Escherichia coli

dc.creatorRey Castellanos, Natalia
dc.creatorStashenko, Elena E.
dc.creatorEstévez Castro, Carlos F.
dc.date2019-04-01T03:51:45Z
dc.date2019-04-01T03:51:45Z
dc.date2015-09
dc.dateinfo:eu-repo/date/embargoEnd/2024-01-31
dc.date.accessioned2022-12-07T14:46:23Z
dc.date.available2022-12-07T14:46:23Z
dc.identifierhttp://repositorio.colciencias.gov.co/handle/11146/34183
dc.identifierContiene 8 referencias bibliográficas. Véase documento adjunto
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5300903
dc.descriptionThe objective of this work was to evaluate DNA protective properties against UVR-induced genotoxicity of an L. origanoides extract obtained by CO2-supercritical fluid extraction and its major constituent, pinocembrin. L. origanoides extract composition was determined by gas chromatography-mass spectrometry (GC-MS) and its antigenotoxic effect using the SOS chromotest. Fluorescence microscopy analysis was also used in cell treatments in order to determine interferences with cell division and different nucleoid shapes categories were measured. The principal constituents of the plant extract were pinocembrin (54.9%), trans-β- caryophyllene (10.9%), carvacrol (6.3%), p-cimene (6.2%), thymol (4.6%), and 1,8-cineole (3.5%). L. origanoides extract protected bacterial cells against UVR-induced genotoxicity at doses between 0.016 and 1 mg/mL; this effect was related to its major constituent pinocembrin. Fluorescence microscopy analysis showed that pinocembrin modulated the cell division and nucleoid shape in E. coli cells. The results were discussed in relation to the DNA protective potential of the L. origanoides species.
dc.descriptionDepartamento Administrativo de Ciencia, Tecnología e Innovación [CO] Colciencias
dc.description5507-543-31904
dc.descriptionPrograma: Bioprospección y desarrollo de ingredientes naturales para las industrias cosmética, farmacéutica y de productos de aseo con base en la biodiversidad colombiana
dc.descriptionno
dc.formatpdf
dc.format19 páginas
dc.formatapplication/pdf
dc.languageeng
dc.relationPrograma: Bioprospección y desarrollo de ingredientes naturales para las industrias cosmética, farmacéutica y de productos de aseo con base en la biodiversidad colombiana. La publicación completa está disponible en : <a href="http://repositorio.colciencias.gov.co/handle/11146/34163" target="blank">http://repositorio.colciencias.gov.co/handle/11146/34163</a>
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.sourceJournal: Natural Product Research
dc.subjectEscherichia coli
dc.subjectBiología vegetal
dc.subjectCromatografía de gases
dc.subjectAntigenotoxicity
dc.subjectSOS Chromotest
dc.subjectUltraviolet radiation
dc.subjectLippia origanoides
dc.subjectSupercritical fluid extraction
dc.subjectPinocembrin
dc.subjectBiología molecular
dc.subjectAceites esenciales
dc.subjectBioquímica vegetal
dc.subjectTecnología química
dc.subjectCatalizadores
dc.titleLippia origanoides supercritical fluid extract and its major constituent pinocembrin diminish ultraviolet radiation-induced DNA damage and modulate cell division in Escherichia coli
dc.typeinfo:eu-repo/semantics/other.generacióndecontenidos
dc.typeinfo:eu-repo/semantics/draft
dc.coverageColombia


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