Lipossoma revestido com hidroxiapatita para o carreamento de fármacos

Abstract

The objective of this work was the development of a hydroxyapatite-coated liposomal system that encapsulated quantum dots and carried drug molecules (bupivacaine hydrochloride) to form a teragnostic liposome system. According to the literature, systems like this one have advantages over conventional ones: greater control of the drug release rate, are stealthier (hydroxyapatite coating) and raise the possibility of monitoring its distribution in vivo (quantum dots). Liposomes were prepared by injection technique, and the synthesis route by co-precipitation of phosphate and calcium precursors obtained the. The increase in the diameter of the liposome particles and the decrease in zeta potential in comparison between samples seems to corroborate the hypothesis that a coating layer was formed. The results of the X-ray diffraction and infrared spectroscopy techniques indicate that the formed coating presents predominantly the presence of the hydroxyapatite phase. Photoluminescence tests confirmed the presence of the liposome-encapsulated quantum dot CdSe, whereas the encapsulation of bupivacaine hydrochloride by the liposomes was confirmed by the nuclear magnetic resonance technique. In addition to these assays, the encapsulation efficiency of the local anesthetic bupivacaine hydrochloride and the drug release profile were also calculated.