| dc.creator | Añez Reverol, Nestor Oswaldo | |
| dc.creator | Crisante R., Gladys E. | |
| dc.creator | Rojas E., Agustina del V. | |
| dc.date | 2009-11-09T17:02:06Z | |
| dc.date | 2009-11-09T17:02:06Z | |
| dc.date | 2007-07 | |
| dc.date.accessioned | 2022-11-16T19:25:38Z | |
| dc.date.available | 2022-11-16T19:25:38Z | |
| dc.identifier | http://www.saber.ula.ve/handle/123456789/29711 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/5203722 | |
| dc.description | Three serological tests (ELISA, IFAT, DAT) were evaluated using sera from selected
individuals with different American cutaneous leishmaniasis (ACL) clinical conditions. Reactivity in
at least 2 of the 3 named tests was established as a criterion for declaring a patient as sero-positive.
Prior to serological testing, people were diagnosed by clinical (presence of lesion = pl or scar=Sc),
parasitological (presence of parasites =pp), immunological (LST) and molecular (PCR) methods. For
a statistical comparison of the evaluated tests 4 groups of people were made up: 1) patients with
active leishmanial lesion (n=44; pl, pp, +LST, +PCR); 2) patients who had recovered from leishmanial
infection (n=43; Sc, +LST, +PCR); 3) asymptomatic individuals from endemic areas showing evidence
of having contacted Leishmania-parasites (n=40; +LST, +PCR) and 4) Leishmania-negative people
from the above localities where leishmaniasis is endemic and living under the same risk conditions,
considered as healthy controls (n=104; -LST, -PCR). Considering the above-established criteria
for sero-positives, the analysis of the results obtained with the 3 tests employed revealed very low
sensitivity values. Seropositive figures of 50% were recorded for the first group, 9% for the second
group, and 12.5% for the third group. Statistical analysis also revealed a low positive predictive value
(PPV=0.73), a low negative predictive value (NPV=0.49), and a low kappa coefficient value (k=0.114).
The present results raise questions about the use of the 3 conventional serological tests compared
here to detect ACL at any clinical condition. | |
| dc.description | 55-62 | |
| dc.description | nanes@ula.ve | |
| dc.description | gecr68@hotmail.com | |
| dc.description | agustinarojas@yahoo.com | |
| dc.format | application/pdf | |
| dc.language | en | |
| dc.publisher | Boletín de malariología y salud ambiental. Vol. XLVII, Nº 1, enero-julio, 2007 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/3.0/ve/ | |
| dc.subject | Facultad de Ciencias | |
| dc.subject | American cutaneous leishmaniasis | |
| dc.subject | Diagnosis | |
| dc.subject | Serology | |
| dc.subject | Artículos | |
| dc.title | Evaluation of conventional serological tests for the diagnosis of American cutaneous Leishmaniasis | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | Texto | |
