Article
Effect of necrosectomy and vacuum-assisted closure (VAC) on mitochondrial function and oxidative stress markers in severe acute pancreatitis
Fecha
2013Autor
Leyva-Porras, C.
Esneider-Alcala, M.A.
Toxqui-Teran, A.
Marquez-Lucero, A.
Aguilar-Martinez, J.A.
Institución
Resumen
Injection molding is a process employed worldwide to manufacture polymer parts. The final properties of the molded part largely depend on the processing conditions used during the manufacturing process. Therefore, it is necessary to develop empirical approaches that help to understand the relationship between the processing conditions and the final properties of the polymer. In this paper we study the effect of the processing conditions of the injection molding process on the Young's modulus of a low-density polyethylene (LDPE). The effect of both the barrel temperature and the mold temperature was investigated using analysis of variance (ANOVA) and the effect of the levels of each parameter was examined using the surface response methodology (SRM). The ANOVA results showed that the mold temperature is the parameter that most significantly impacts the Young's modulus, followed by the barrel temperature, while the combined interaction of both is negligible. SRM showed that the Young's modulus increases with the mold temperature and decreases with the barrel temperature. Based on the SRM, an empirical equation is proposed which can be used to predict the modulus employing only the barrel and mold temperatures. The changes in the microstructure of the injection molded part are discussed in terms of the crystallinity degree. All this was corroborated with X-ray diffraction (XRD) and differential scanning calorimetry (DSC). " 2013 American Chemical Society.",,,,,,"10.1021/ie3032422",,,"http://hdl.handle.net/20.500.12104/40963","http://www.scopus.com/inward/record.url?eid=2-s2.0-84876514821&partnerID=40&md5=b963ffc547df731df73a3a1e1832621f http://dx.doi.org/10.1021/ie3032422",,,,,,"16",,"Industrial and Engineering Chemistry Research",,"5666 5671",,"52",,"Scopus ChemAbsS WOS",,,,,,,,,,,,"Effect of molding parameters on young's modulus of an injection molded low-density polyethylene (LDPE)",,"Article"
"42746","123456789/35008",,"Miranda-Díaz, A.G., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Hermosillo-Sandoval, J.M., Department of General Surgery, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro SocialGuadalajara, Jalisco, Mexico; Gutiérrez-Martínez, C.A., Intensive Care Unit. Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro SocialGuadalajara, Jalisco, Mexico; Rodríguez-Carrizalez, A.D., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Roman-Pintos, L.M., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Cardona-Muñoz, E.G., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Pacheco-Moisés, F.P., Department of Chemistry, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Arias-Carvajal, ., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico",,"Miranda-Diaz, A.G. Hermosillo-Sandoval, J.M. Gutierrez-Martinez, C.A. Rodriguez-Carrizalez, A.D. Roman-Pintos, L.M. Cardona-Munoz, E.G. Pacheco-Moises, F.P. Arias-Carvajal, O.",,"2014",,"Background: Severe acute pancreatitis (SAP) is associated with high morbidity and mortality. Objective: To evaluate whether necrosectomy, alone or combined with vacuum-assisted closure (VAC), has any additional beneficial effects on mitochondrial function and/or oxidative stress markers in SAP. Methods: Patients with SAP, APACHE II score > 8, and inadequate response to management in an intensive care unit were included in a prospective observational study. Sixteen underwent necrosectomy and 24 underwent necrosectomy plus VAC every 48 h. Patients were then categorized as survivors or deceased. Submitochondrial membrane fluidity of platelets and F<inf>0</inf>F<inf>1</inf>-ATPase hydrolysis were measured to represent mitochondrial function. Oxidative/nitrosative stress was measured using lipoperoxides (LPOs), nitric oxide (NO), erythrocyte membrane fluidity, and total antioxidant capacity (TAC). Results: Membrane fluidity in submitochondrial particles of platelets remained significantly increased throughout the study, and then eventually rised in deceased patients managed with necrosectomy + VAC vs. survivors (p < 0.041). Hydrolysis was significantly increased from baseline to endpoint in all patients, predominating in those who died after management with necrosectomy (p < 0.03). LPO increased in all patients, and necrosectomy was more efficient for the eventual decrease in survivors (p < 0.039). NO was found to be increased for the baseline-endpoint result among both survivors and deceased patients with both management options. Erythrocyte membrane fluidity was increased in survivors managed with necrosectomy + VAC, and eventually returned to normal (p < 0.045). TAC was found to be consumed in all patients for the duration of the study. Conclusions: Mitochondrial dysfunction and oxidative?nitrosative stress with significant systemic antioxidant consumption were found. Necrosectomy was more efficient and better cleared LPOs. Necrosectomy + VAC improved erythrocyte membrane fluidity and increased survival. " 2014 Arán Ediciones, S. L.