Article
Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
Fecha
2013Registro en:
10.1590/S1415-47572014000100016
Autor
Ortiz, M.
Campos, L.
Berrios, F.
Rodriguez, F.
Hermosillo, B.
Gonzalez, J.
Institución
Resumen
Multispecies quantitative and qualitative models of the kelp Lessonia nigrescens and the tunicate Pyura praeputialis were constructed for intertidal areas of northern Chile (SE Pacific). Information on biomass, P/B ratios, catches, food spectrum, consumption and dynamics of commercial and non-commercial species was obtained and examined using Ecopath with Ecosim and Loop Analysis theoretical frameworks. The biomass of L. nigrescens and P. praeputialis constituted the most important compartments, exceeding 97% of the total biomass in each model system. Based on Pp/R, the system of P. praeputialis appeared to be the most developed. However, according to Pp/B, A/C, Ai/Ci, and redundancy, the L. nigrescens system was the most developed and, in turn, the least resistant to disturbances. The results obtained using mixed trophic impacts (MTI), Ecosim simulations, and system recovery time (SRT) showed different response patterns. The tunicate species propagated higher effects on the remaining species, whereas the kelp species presented the longest SRT (as a resilience measure). The model keystone species indices suggested that each model system contained a core of ecologically related species. In the L. nigrescens system, core was made up of the filter feeders Semimytilus algosus, barnacles, and small epifauna herbivores (SEH) and the predators Concholepas concholepas and Heliaster helianthus. In the P. praeputialis system, the core consisted of phyoplankton, zooplankton, other filter feeders and the predators C. concholepas, H. helianthus, other starfish, and large epifauna. The outcomes obtained in the current work did not indicate that the alien tunicate P. praeputialis was a better or superior bio-engineer when compared to the system constructed by the kelp L. nigrescens. Rather, each species was relevant and relied on different ecological mechanisms. " 2012 Elsevier B.V.",,,,,,"10.1016/j.ecolmodel.2012.10.014",,,"http://hdl.handle.net/20.500.12104/43057","http://www.scopus.com/inward/record.url?eid=2-s2.0-84871405705&partnerID=40&md5=2c2b584cbe02b800e6335a783e219c3d",,,,,,,,"Ecological Modelling",,"307 318",,"250",,"Scopus WOS",,,,,,"Ecopath; Ecosim; Lessonia nigrescens; Loop Analysis model keystone indices; Pyura praeputialis",,,,,,"Network properties and keystoneness assessment in different intertidal communities dominated by two ecosystem engineer species (SE Pacific coast): A comparative analysis",,"Article"
"42053","123456789/35008",,"Sossa-Azuela, J.H., Centro de Investigación en Computación-Instituto Politécnico Nacional, Av. Juan de Dios Bátiz s/n, Mexico, DF, Mexico; Santiago-Montero, R., Instituto Tecnológico de León, Av. Tecnológico S/N, Frac. Julian-de Obregon, Leon-Guanajuato, Mexico; Pérez-Cisneros, M., Departamento de Ciencias Computacionales, Universidad de Guadalajara, CUCEI Av. Revolución 1500, Guadalajara, Jalisco, Mexico; Rubio-Espino, E., Centro de Investigación en Computación-Instituto Politécnico Nacional, Av. Juan de Dios Bátiz s/n, Mexico, DF, Mexico",,"Sossa-Azuela, J.H. Santiago-Montero, R. Perez-Cisneros, M. Rubio-Espino, E.",,"2013",,"We describe a method to compute the Euler number of a binary digital image based on a codification of contour pixels of the image's shapes. The overall procedure evolves from a set of lemmas and theorems, their demonstration and their numerical validation. The method is supported through an experimental set which analyzes some digital images and their outcome to demonstrate the applicability of the procedure. The paper also includes a discussion about present and futures steps on this research.",,,,,,,,,"http://hdl.handle.net/20.500.12104/40274","http://www.scopus.com/inward/record.url?eid=2-s2.0-84881178632&partnerID=40&md5=bb67f49fca2bd4db6f9b58f095a40c56 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1665-64232013000300006&lng=es&nrm=iso&tlng=en; http://132.248.9.1:8991/F/QYBFCDEMMK86QP7AN8HS2AYCPJC21Q8TXQAKE7H5DVGALAVUKF-08765?func=full-set-set&set_number=029909&set_entry=000073&format=999",,,,,,"3",,"Journal of Applied Research and Technology",,"360 370",,"11",,"Scopus WOS ScIELO PERIODICA",,,,,,"Binary shape description; Euler number; Topological descriptor; Topological invariant / Engineering, Electrical & Electronic; Engineering, Multidisciplinary",,"Procesamiento de datos",,,,"Computing the euler number of a binary image based on a vertex codification",,"Article"
"42012","123456789/35008",,"Alvarez-Moya, C., Environmental Mutagenesis Laboratory, Departamento de Biología Celular y Molecular, University of Guadalajara, Zapopan, Mexico; Silva, M.R., Environmental Mutagenesis Laboratory, Departamento de Biología Celular y Molecular, University of Guadalajara, Zapopan, Mexico; Valdez Ramírez, C., Environmental Mutagenesis Laboratory, Departamento de Biología Celular y Molecular, University of Guadalajara, Zapopan, Mexico; Gallardo, D.G., Environmental Mutagenesis Laboratory, Departamento de Biología Celular y Molecular, University of Guadalajara, Zapopan, Mexico; León Sánchez, R., Banco Nacional Genúmico de Tilapia, Departamento de Ingeniería de Proyectos, University of Guadalajara, Guadalajara, Mexico; Aguirre, A.C., Unit of Medical and Pharmaceutical Biotechnology, Jalisco Center for Research and Assistance in Technology and Design, Guadalajara, Mexico; Velasco, A.F., División de Ciencias Biológicas, University of Guadalajara, Zapopan, Mexico",,"Alvarez-Moya, C. Silva, M.R. Valdez Ramirez, C. Gallardo, D.G. Leon Sanchez, R. Aguirre, A.C. Velasco, A.F.",,"2014",,"There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-oxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 ?M) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at 7 ?M, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at 0.7 ?M. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7-7 ?M. " 2014, Sociedade Brasileira de Genética. Printed in Brazil.