Article
Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures
Fecha
2007Autor
Munoz-Munoz, A.C.
Gutierrez-Pulido, H.
Rodriguez-Dominguez, J.M.
Gutierrez-Mora, A.
Rodriguez-Garay, B.
Cervantes-Martinez, J.
Institución
Resumen
We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690/F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saint-paulia ionantha. © 2007 Optical Society of America.