Article
Progesterone fate in rabbit cornea
Fecha
1987Autor
Navarro-Ruiz, A.
Garcia-Estrada, J.
Almodovar-Cuevas, C.
Bastidas-Ramirez, B.E.
Roman-Maldonado, S.
Garzon, P.
Institución
Resumen
1. 1. Excised cornea from adult New Zealand rabbits were incubated with progesterone-4-14C in Eagle's media for 96 hr. Samples were inactivated at intervals of 24 hr incubation periods. The following metabolites of progesterone were isolated: 2. 2. 20?-Hydroxy-4-pregnen-3-one, 20-hydroxy-4-pregnen-3-one, 5?-pregnane-3,20-dione; 5?-pregnane-3,20-dione and 6?-hydroxy-4-pregnen-3,20-dione. 3. 3. 20?-Hydroxy-pregnen-3-one was the predominant metabolite of progesterone-4-14C. A linear increase was observed throughout 96 hr. The opposite was found for 5? and 5? pregnane-3,20-dione. 4. 4. Compounds remaining at the origin of the paper chromatograms contained 6?-hydroxy-4-pregnen-3,20-dione and other still unidentified metabolites of progesterone-4-14C. 5. 5. Presence of 20? and 20?-reductase; 5? and 5?-reductase and 6?-hydroxylase enzyme systems are involved in corneal progesterone metabolism. 6. 6. No fungal neither bacterial enzymatic biotransformation occurred in the culture media. � 1987.