HIV infection can reduce CD4 lymphocyte count, which may lead to opportunistic infections such as tuberculosis. Lymphadenopathy is usually caused by tuberculosis and shows various cytological features. This study aims to determine the association between cytology features of tuberculous lymphadenopathy and CD4 serum level in HIV patients. This analytical study involves HIV patients with lymphadenopathy in Medan. Cytology features are acquired from cytology examination and CD4 serum level.