The crude ethanolic extract form the leaves of hamelia patens Jacq. was active in vitro againts Leishmania mexicana. Partititon of the extract, showed that the activity was confined to the dichloromethane extrac. The four alkaloids isolated from this extract: isopteropodine (1), palmirine (2), rumberine (3) and mitrajavine (4) were evaluated in vitro for antileishmanial activity with IC 26 microgramos, the alkaloid mitrajavine (4) and two flavonoids isolated from the ethyl acetate extract: kaempferol-3-O-rutinoside (5), and (-) epicatechine (6) are reported for the first time in this plant.