| dc.creator | Martinkovic, Franjo | |
| dc.creator | Matanović, KRESIMIR | |
| dc.creator | Rodrigues, Adriana | |
| dc.creator | Garcia, Herakles | |
| dc.creator | Teixeira, Marta M | |
| dc.date | 2013-10-13T05:49:13Z | |
| dc.date | 2013-10-13T05:49:13Z | |
| dc.date | 2013-10-13 | |
| dc.date.accessioned | 2022-10-28T00:55:06Z | |
| dc.date.available | 2022-10-28T00:55:06Z | |
| dc.identifier | 1550-7408 | |
| dc.identifier | http://hdl.handle.net/10872/4408 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4938717 | |
| dc.description | Trypanosoma (Megatrypanum) melophagium is a parasite of sheep transmitted by sheep keds, the sheep-restricted
ectoparasite Melophagus ovinus (Diptera: Hippoboscidae). Sheep keds were 100% prevalent in sheep from five organic farms in
Croatia, Southeastern Europe, whereas trypanosomes morphologically compatible with T. melophagium were 86% prevalent in the
guts of the sheep keds. Multilocus phylogenetic analyses using sequences of small subunit rRNA, glycosomal glyceraldehyde-3-
phosphate dehydrogenase, spliced leader, and internal transcribed spacer 1 of the rDNA distinguished T. melophagium from all
allied trypanosomes from other ruminant species and placed the trypanosome in the subgenus Megatrypanum. Trypanosomes from
sheep keds from Croatia and Scotland, the only available isolates for comparison, shared identical sequences. All biologic and
phylogenetic inferences support the restriction of T. melophagium to sheep and, especially, to the sheep keds. The comparison of
trypanosomes from sheep, cattle, and deer from the same country, which was never achieved before this work, strongly supported
the host-restricted specificity of trypanosomes of the subgenus Megatrypanum. Our findings indicate that with the expansion of
organic farms, both sheep keds and T. melophagium may re-emerge as parasitic infections of sheep. | |
| dc.description | We thank the support provided by the Faculty of Veterinary
Medicine, University of Zagreb in Croatia. [Correction made
here after initial online publication.]. We are grateful to Marta
Campaner for cultures of trypanosomes, Carmen S. A. Takata
for help with the morphological data, and Ma´ rcio M. Yamamoto
for sequencing support. We are grateful to Prof. Erney P.
Camargo for critical revision of the manuscript. This work was
partially supported by grants from the Brazilian agency CNPq.
Adriana C. Rodrigues is a postdoctoral fellow of PNPDCAPES
in Brazil, and Herakles A. Garcia was recipient of
scholarships from CDCH-UCV in Venezuela | |
| dc.language | en | |
| dc.relation | Journal of Eukaryotic Microbiology;2012; 59: 134-44 | |
| dc.subject | Evolution, host-specificity, molecular taxonomy, morphology, phylogeny, artiodactyls, Trypanosoma theileri | |
| dc.title | Trypanosoma (Megatrypanum) melophagium in the Sheep Ked Melophagus ovinus from Organic Farms in Croatia: Phylogenetic Inferences Support Restriction to Sheep and Sheep Keds and Close Relationship with Trypanosomes from Other Ruminant Species | |
| dc.type | Article | |
