Eficiencia de la multiplicación in vitro de Juglans neotrópica (nogal) en Biorreactores de Inmersión Temporal (BIT)

Abstract

The Andean walnut (Juglans neotropica) is a species prized mainly for its wood, dyes and fruits with a great adaptive capacity to be used in afforestation and reforestation programs. Generally propagated by seeds, this species presents limitations during its germination due to the deep dormancy of the embryos that delays germination. Currently, there is great interest in developing in vitro propagation protocols that could allow to reduce its germination time. In order to evaluate the efficiency of Temporal Immersion Bioreactors (BIT) in walnut multiplication as a novel tool for the accelerated production of plants, this study compares the results of in vitro multiplication in BIT versus the conventional method using semisolid medium, obtaining greater efficiency in the BIT system. Three immersion frequencies were tested (every 6, 8 and 12 hours) along with the application of forced ventilation, during a period of 45 days. A comparative costs analysis was also carried out for both protocols. The frequency of immersion showing the best results in relation to the quality of shoots generated was immersion for 12 h and forced ventilation for one minute every 4 hours. This treatment did not have change the multiplication rate but reduced the hyperhydricity of the shoots formed.