masterThesis
Efectos de la avidez de anticuerpos lgG sobre la tasa de transferencia congénita de neospora caninum en vacas lecheras
Fecha
2017Autor
Taboada Pico, Mentor Guillermo
Institución
Resumen
The objective of this research was: To determine the congenital transfer rate of Neospora caninum, in seropositive cows, according to the avidity of the IgG antibodies. It was carried out in three dairy herds in the provinces of Azuay and Cañar, in the southern highlands of Ecuador, Where abortions and reproductive problems have been reported, 148 dairy cows were selected, pregnant in the last third of gestation, Their serological status was determined by indirect ELISA enzyme immunoassay, to obtain the 40 cows positive to Neospora Caninum, Which would form part of the study. To determine the avidity of the IgG antibodies, the protocol was modified to each cow of the 40 will be performed another ELISA, With the modified protocol for avidity. To the blood of calves will be carried out 2 ELISA tests with the normal protocol of affinity and avidity. This will allow to know the protective effect of the natural antibodies in dairy cattle with high and low avidity against the vertical transmission of the parasite in the conditions of production of the Ecuadorian sierra.