dc.creator | Vallecillo Maza, Antonio Javier | |
dc.date.accessioned | 2018-01-11T16:47:57Z | |
dc.date.accessioned | 2022-10-20T21:43:13Z | |
dc.date.available | 2018-01-11T16:47:57Z | |
dc.date.available | 2022-10-20T21:43:13Z | |
dc.date.created | 2018-01-11T16:47:57Z | |
dc.date.issued | 2016-01-01 | |
dc.identifier | 0022149X | |
dc.identifier | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84951744947&doi=10.1017%2fS0022149X14000686&partnerID=40&md5=38dce7a2ff053f319971250ad0013a39 | |
dc.identifier | http://dspace.ucuenca.edu.ec/handle/123456789/29284 | |
dc.identifier | 10.1017/S0022149X14000686 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4605331 | |
dc.description.abstract | Leucine aminopeptidase (LAP) and cathepsin L1 (CL1) are important enzymes for the pathogenesis and physiology of Fasciola hepatica. These enzymes were analysed in silico to design a chimeric protein containing the most antigenic sequences of LAP (GenBank; AAV59016.1; amino acids 192-281) and CL1 (GenBank CAC12806.1; amino acids 173-309). The cloned 681-bp chimeric fragment (rFhLAP-CL1) contains 270 bp from LAP and 411 bp from CL1, comprising three epitopes, DGRVVHLKY (amino acids 54-62) from LAP, VTGYYTVHSGSEVELKNLV (amino acids 119-137) and YQSQTCLPF (amino acids 161-169) from CL1. The ?25 kDa rFhLAP-CL1 chimeric protein was expressed from the pET15b plasmid in the Rosetta (DE3) Escherichia coli strain. The chimeric protein rFhLAP-CL1, which showed antigenic and immunogenic properties, was recognized in Western blot assays using F. hepatica-positive bovine sera, and induced strong, specific antibody responses following immunization in rabbits. The newly generated chimeric protein may be used as a diagnostic tool for detection of antibodies against F. hepatica in bovine sera and as an immunogen to induce protection against bovine fasciolosis. | |
dc.language | en_US | |
dc.publisher | CAMBRIDGE UNIVERSITY PRESS | |
dc.source | Journal of Helminthology | |
dc.title | Construction and evaluation of a chimeric protein made from Fasciola hepatica leucine aminopeptidase and cathepsin L1 | |
dc.type | Article | |