bachelorThesis
Estandarización del método de bioautografía directa como estrategia para la evaluación de extractos naturales como antimicrobianos frente a Escherichia coli y Staphylococcus aureus
Fecha
2022-10-12Autor
Aguirre Loaiza, Daniela Alejandra
Coraizaca Guzmán, Christian Javier
Institución
Resumen
Direct bioautography is a fast and simple method that has the advantage of detecting
antimicrobial activity on chromatographic plates, allowing us to directly observe the
fractions showing such activity. The present work consisted of the standardization of the
direct bioautography method, by using both, polar and nonpolar plant extracts, with
antimicrobial activity against Escherichia coli and Staphylococcus aureus. In addition, for
thin layer chromatography (TLC), eluents were used according to the polarity of the plant
extracts, which in turn were also compatible with the antibiotics gentamicin and ampicillin,
used as positive controls. By means of this technique, it was possible to observe the
growth of S. aureus in the TLC plates, however, after several tests, it was not possible to
visualize the E. coli growth, therefore, it was decided to implement the bioautography
method by immersion or coating, obtaining as a result the growth of both, bacteria and
the visualization of the inhibition zones for the antibiotics and certain extracts by means
of the use of MTT and resazurin. However, due to the instability of this method because
of the different variables that must be controlled, the objective of standardizing
bioautography as a tool for evaluating the antibacterial potential of natural extracts was
not achieved.