Article
Evaluación de los sistemas de cultivo semisólido y BIT en la multiplicación in vitro de Juglans neotrópica
Fecha
2017Autor
Rocano, Mélida N.
Villena, Paulina G.
Peña, Denisse F.
Institución
Resumen
The purpose of this study was to evaluate two culture systems, semi-solid and temporary immersion in
bioreactors (BIT), for the micropropagation of Juglans neotropica, using zygotic embryos. Additionally,
in the BIT culture system also the effect of the frequency of immersion and forced aeration on the
micropropagation was examined. The culture growth of the shoots was measured by the following
characteristics: number and size of the shoots, number of leaves, and the wet and dry weight. No
statistical differences were detected. In general, the BIT immersion system produced an equivalent
number of plants of similar characteristics as the embryos propagated in the traditional semi-solid
medium method. However, BITs produced more shoots, forced ventilation resulted in less hyperhydric
shoots and the BIT system was considerable cheaper, resulting in an average production cost of $0.21
per plant versus $0.40 in the semi-solid propagation method.