Evaluación de la calidad de ovocitos provenientes de vaconas criollas y ovarios de matadero

Abstract

This research work was conducted at the experimental farm "Irquis", Faculty of Agricultural Sciences at the University of Cuenca, the aim of this study was to evaluate the quality of oocytes in cattle recovered from two sources: transvaginal follicular aspiration guided by ultrasound (OPU) in native cattle and follicular aspiration in abattoir ovaries. 10 cyclical indigenous heifers from 3 to 3.5 CC maintained on pasture and supply of mineral salts were used; and 200 slaughterhouse ovaries. Oocyte recovery OPU and follicular aspiration was performed with disposable needles 18G and a vacuum pressure of 90 mmHg. The oocytes obtained were classified into three categories according to Hawk & Wall (1994). The morphometry was made using a high definition camera mounted on an inverted lens 10X and equipped with software V.3.7 AmScope microscope. The viability was assessed with trypan blue and enzyme activity with brilliant cresyl blue test (BCB). For statistical analysis the Chi-square and statistical "Mann Whitney" (P <0.05) was used. The results show significant differences for sources of oocytes (P <0.05) while for the viability and enzyme activity no significant differences (P> 0.05). It was concluded that oocyte recovery sources produce different qualities but oocyte viability and similar enzyme activity