SRPKs regulate aggregation of ELKS2 and modulates synaptogenesis in dissociated hippocampal neurons
Fecha
20162016
Institución
Resumen
ELKS2 is an active zone protein (AZ) that forms
macromolecular complexes with other proteins of the
cytomatrix of the AZ through the interaction with Munc-13,
RIM1, Piccolo and Bassoon. Due to this multiple interactions
ELKS2 is considered a central organizer of the presynaptic
scaffold.
The putative ELKS2 homologous in D. melanogaster
Bruchpilot (BRP), is critical for the functional assembly of the
AZ and modulates the efficacy of neurotransmitter release. In
the absence of the serine-arginine (SR) protein kinase
SRPK79D BRP tends to aggregate ectopically along axons
and T-bars are disrupted. In vertebrates three members of
SRPKs have been described (SRPK1-3), however there is not
report about a putative function in synaptic assembly.
In this work we explore the effect of the kinases SRPK on the
clustering properties of ELKS2 both in heterologous cells and
hippocampal neurons in culture.